Kinetics of Conversion of Prothrombin to Thrombin by Biological Activators
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چکیده
منابع مشابه
Kinetics of Conversion of Prothrombin to Thrombin by Biological Activators I. DEMONSTRATION OF A PROTHROMBIN DERIVATIVE WHICH REACTS WITH PROTEOLYTIC
Purified prothrombin can be converted into thrombin in different ways (2), namely, by the use of biological activators, by the action of high concentrations of sodium citrate, and by treatment with trypsin. Biological activation of prothrombin requires lipoprotein tissue-factor(s) that have prothrombin-converting or thromboplastic activity, protein serum-factor(s) which augment thromboplastic a...
متن کاملKinetics of conversion of prothrombin to thrombin by biological activators. I. Demonstration of a prothrombin derivative which reacts with proteolytic enzyme inhibitors.
Purified prothrombin can be converted into thrombin in different ways (2), namely, by the use of biological activators, by the action of high concentrations of sodium citrate, and by treatment with trypsin. Biological activation of prothrombin requires lipoprotein tissue-factor(s) that have prothrombin-converting or thromboplastic activity, protein serum-factor(s) which augment thromboplastic a...
متن کاملThe Conversion of Prothrombin to Thrombin
Prothrombin is activated by activated Factor X f (Factor V and phospholipid) in the presence of diisopropylfluorophosphate which preferentially inhibits thrombin. Under these conditions a single activation fragment, designated Fragment 1.2, is released which represents the complete nonthrombin portion of the prothrombin molecule. The same fragment is seen as a transient species in the absence o...
متن کاملThe Conversion of Prothrombin to Thrombin V. THE ACTIVATION OF PROTHROMBIN BY FACTOR X, IN THE PRESENCE OF PHOSPHOLIPID*
The direct and concomitant formation of Intermediate 2 and Fragment 1.2 was demonstrated by activating prothrombin in the presence of iPrzP-F. In the absence of this inhibitor, thrombin, in addition to catalyzing the reaction of Equation 2, cleaves Fragment 1 from prothrombin and in so doing eliminates the possibility of observing phospholipid catalysis from all but the earliest stages of react...
متن کاملThe conversion of prothrombin to thrombin. V. The activation of prothrombin by factor Xa in the presence of phospholipid.
The direct and concomitant formation of Intermediate 2 and Fragment 1.2 was demonstrated by activating prothrombin in the presence of iPrzP-F. In the absence of this inhibitor, thrombin, in addition to catalyzing the reaction of Equation 2, cleaves Fragment 1 from prothrombin and in so doing eliminates the possibility of observing phospholipid catalysis from all but the earliest stages of react...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1963
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)83976-5